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http://hdl.handle.net/10314/3101
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Título: | A Rapid and Sensitive HPLC–DAD Assay to Quantify Lamotrigine, Phenytoin and Its Main Metabolite in Samples of Cultured HepaRG Cells |
Autores: | Ferreira, Ana Rodrigues, Márcio Falcão, Amílcar Alves, Gilberto |
Palavras Chave: | Carbamazepine Oxcarbazepine HepaRG cells High-performance liquid chromatography In vitro metabolism and drug interaction studies |
Data: | May-2016 |
Editora: | Journal of Chromatographic Science |
Resumo: | A sensitive and fast high-performance liquid chromatography-diode-array detection assay was developed and validated for the simultaneous quantification of 5-(4-hydroxyphenyl)-5-phenylhydantoin (HPPH), phenytoin (PHT) and lamotrigine (LTG) in samples of cultured HepaRG cells. Chromatographic separation of analytes and internal standard (IS) was achieved in ∼15 min on a C18-column, at 35°C, using acetonitrile (6%), methanol (25%) and a mixture (69%) of water-triethylamine (99.7:0.3, v/v; pH 6.0), pumped at 1 mL/min. The analytes and IS were detected at 215 or 235 nm. Calibration curves were linear with regression coefficients >0.994 over the concentration ranges of 0.1-15 µg/mL for HPPH; 0.15-30 µg/mL for PHT and 0.2-20 µg/mL for LTG. The method showed to be accurate (bias value of ±10.5 or ±17.6% in the lower limit of quantification, LLOQ) and precise (coefficient variation ≤8.1 or ≤15.4% in the LLOQ), and the absolute recovery of the analytes ranged from 62.5 to 96.9%. HepaRG cells have emerged as a very promising in vitro model to evaluate metabolic, drug interaction and/or pharmacokinetic studies, and this methodology will be suitable to support subsequent studies involving the antiepileptic drugs PHT and LTG. |
URI: | http://hdl.handle.net/10314/3101 |
ISSN: | 1612-1112 |
Aparece nas Colecções: | Artigos em Revista Internacional (ESS)
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Ficheiros deste Registo:
Ficheiro |
Descrição |
Tamanho | Formato |
Rodrigues, Márcio - A Rapid and Sensitive HPLC–DAD Assay to Quantify Lamotrigine.pdf | | 294Kb | Adobe PDF | Ver/Abrir | |
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