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| Título: | HPLC–DAD Method for the Quantification of Carbamazepine, Oxcarbazepine and their Active Metabolites in HepaRG Cell Culture Samples |
| Autores: | Ferreira, Ana
Rodrigues, Márcio
Falcão, Amílcar
Alves, Gilberto |
| Palavras Chave: | Carbamazepine
Oxcarbazepine
HepaRG cells
High-performance liquid chromatography
In vitro metabolism and drug interaction studies |
| Data: | Mar-2016 |
| Editora: | Springer-Verlag Berlin Heidelberg |
| Resumo: | A new, sensitive and fast high-performance liquid chromatography–diode-array detection assay is herein reported, for the first time, to simultaneously quantify carbamazepine (CBZ), oxcarbazepine (OXC), and the active metabolites carbamazepine-10,11-epoxide (CBZ-E) and licarbazepine (LIC) in HepaRG cell culture medium samples. Chromatographic separation of analytes (CBZ, CBZE, OXC, LIC) and internal standard (IS) was achieved in less than 15 min on a C18-column, at 35 °C, using a mobile phase composed of water/methanol/acetonitrile (69:25:6 v/v/v) pumped at 1 mL min−1. The analytes and IS were detected at 215 nm. The method proved to be selective, accurate (bias ± 14.6 %), precise (coefficient of variation ≤13.1 %) and linear (r2 ≥ 0.9901) over the concentration ranges of 0.1–15 μg mL−1 for CBZ; 0.1–5 μg mL−1 for CBZ-E and OXC; and 0.1–40 μg mL−1 for LIC. Furthermore, the absolute recovery of the analytes ranged from 64.5 to 96.9 % and their stability was demonstrated in the studied conditions. This validated HPLC assay will be a suitable tool to support future in vitro metabolism profiling, drug interaction and other pharmacokinetic-based studies in HepaRG cells involving these antiepileptic drugs (CBZ
and OXC) and their main metabolites. |
| URI: | http://hdl.handle.net/10314/3102 |
| Aparece nas Colecções: | Artigos em Revista Internacional (ESS)
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Ficheiros deste Registo:
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Descrição |
Tamanho | Formato |
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| Rodrigues, Márcio - HPLC–DAD Method for the Quantification of Carbamazepine.pdf | | 532Kb | Adobe PDF | Ver/Abrir | |
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