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Título: Development and validation of an analytical methodology for the determination of antipsychotic drugs in hospital wastewaters by gas chromatography–tandem mass spectrometry (GC–MS/MS)
Autores: Logarinho, F.
Rosado, T.
Lourenço, C.
Araújo, André
Data: 2015
Resumo: The consumption of psychiatric drugs has seen a huge increase during the last years as a consequence of the financial European crisis, and this can lead to psychological health effects causing several psychiatric diseases. These drugs have become pseudo-persistent in the environment due to their large volumes of use, and nowadays they are considered environmental emerging contaminants. Within this main group, the antipsychotic class have experienced an expressive increase in consumption, namely in Portugal, being used for the management of psychotic episodes as well as for other related behavioral symptoms and even other therapeutic indications. The present work describes the development and validation of a highly sensitive analytical method for the simultaneous determination of antipsychotic drugs in influent and effluent hospital wastewaters by GC–MS/MS. The studied compounds were levomepromazine, clozapine, chlorpromazine, haloperidol, quetiapine and ciamemazine using promazine as internal standard. Sample preparation was carried out by solid phase extraction (SPE) using mixed mode-columns (Strata XC – 200 mg) and followed by derivatization of the extracts with MSTFA (with TMCS). Chromatographic separation was achieved on a 5% phenylmethylsiloxane column. All chromatographic conditions and mass spectrometric parameters were previously optimized to enhance the maximum signal. The method was validated following internationally accepted criteria, and the studied parameters included selectivity, linearity, limits of detection (LOD) and quantification (LOQ), instrumental limits, precision and accuracy, stability and recovery. The procedure was linear for concentrations ranging from 0.1 to 10 g/L (0.02–2 g/L for haloperidol), with determination coefficients higher than 0.99 for all analytes. Intra- and inter-day precision was lower than 15% for all analytes at the studied concentrations, while accuracy remained between a ±15% interval. Recoveries ranged from 35% to 80%. Low LODs were achieved, between 2 and 10 pg/mL, allowing a reliable and accurate quantification of the analytes at trace level (low ppb). All studied parameters complied with the defined criteria and the method enabled the successful determination of antipsychotics in hospital wastewater samples.
URI: http://hdl.handle.net/10314/3258
Aparece nas Colecções:Artigos em Acta de Conferência Internacional (ESS)

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