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Biblioteca Digital do IPG >
Escola Superior de Saúde (ESS) >
Artigos em Revista Internacional (ESS) >
Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10314/3928
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| Título: | A novel HPLC method for the determination of zonisamide in human plasma using microextraction by packed sorbent optimised by experimental design |
| Autores: | Lourenço, Dina
Sarraguça, Mafalda
Alves, Gilberto
Coutinho, Paula
Araújo, André
Rodrigues, Márcio |
| Palavras Chave: | A novel HPLC method for the determination of zonisamide in human plasma using microextraction by packed sorbent optimised by experimental design |
| Data: | 21-Sep-2017 |
| Editora: | Analytical Methods |
| Resumo: | A novel high-performance liquid chromatography-diode array detection method based on microextraction
by packed sorbent (MEPS) as a sample preparation approach is described for the determination of
zonisamide (ZNS) in human plasma. MEPS parameters were optimised using a Plackett–Burman
experimental design to achieve the best extraction conditions. The chromatographic separation of ZNS
and chloramphenicol [internal standard (IS)] was achieved in less than 5 min on a C18-column, at 35 C,
using a mobile phase composed of acetonitrile/water (35 : 65, v/v) pumped isocratically at 1.0 mL min 1.
ZNS and IS were detected at 240 nm. No endogenous and exogenous interference was observed at the
retention times of the analyte of interest (ZNS) and IS. A good linearity was obtained for ZNS (r2 ¼
0.9960) in the range of 0.2–80 mg mL 1 in plasma. The method was shown to be precise (CV # 13.3%)
and accurate (bias 12.3%), and the absolute recovery ranged from 63.8% to 65.2%. The stability of ZNS
was demonstrated in plasma samples under all predictable handling and storage conditions. The
proposed assay was applied to the analysis of human plasma samples obtained from epilepsy patients
under ZNS therapy, and the results supported its usefulness for therapeutic drug monitoring in clinical
practice. |
| URI: | http://hdl.handle.net/10314/3928 |
| ISSN: | DOI: 10.1039/c7ay01912b |
| Aparece nas Colecções: | Artigos em Revista Internacional (ESS)
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Ficheiros deste Registo:
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Tamanho | Formato |
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| coutinho_353a.pdf | | 637Kb | Adobe PDF | Ver/Abrir | |
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