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Utilize este identificador para referenciar este registo: http://hdl.handle.net/10314/4178

Título: First HPLC method for the simultaneous quantification of levetiracetam, zonisamide, lamotrigine, pentylenetetrazole and pilocarpine in rat plasma and brain
Autores: Fonseca, Beatriz
Rodrigues, Márcio
Alves, Gilberto
Palavras Chave: First HPLC method for the simultaneous quantification of levetiracetam, zonisamide, lamotrigine, pentylenetetrazole and pilocarpine in rat plasma and brain
Data: 31-Jan-2018
Editora: Analytical Methods
Resumo: A new high-performance liquid chromatography-diode-array detection (HPLC-DAD) method is herein reported for the first time for the simultaneous determination of three antiepileptic drugs (AEDs) [levetiracetam (LEV), zonisamide (ZNS) and lamotrigine (LTG)] and two convulsant agents [pentylenetetrazole (PTZ) and pilocarpine (PIL)] in rat plasma and brain matrices. Chromatographic separation of all analytes and 7-hydroxycoumarin, used as internal standard (IS), was achieved in less than 19 min on a C18 column maintained at 30 C using a mobile phase composed of acetonitrile (7.5%) and a mixture (92.5%) of water–triethylamine (99.5 : 0.05, v/v, pH 6.4) pumped at a flow rate of 1 mL min 1. The analytes and the IS were detected at 205, 215 or 240 nm. This method showed to be selective and the calibration curves were linear (r2 $ 0.991) over the concentration ranges of 0.4–100 mg mL 1 for LEV, 2–100 mg mL 1 for PTZ, 0.75–100 mg mL 1 for PIL, 0.15–100 mg mL 1 for ZNS and 0.3–50 mg mL 1 for LTG in both rat matrices. The overall data of precision and accuracy were in accordance with international guidelines on bioanalytical method validation. The analytes were extracted from the rat matrices by a liquid–liquid extraction procedure and the absolute recovery of the analytes was precise and reproducible. Additionally, this novel HPLC-DAD method was applied in a preliminary pharmacokinetic study in rats, which involved the target AEDs and convulsant agents, demonstrating to be a useful bioanalytical tool to support future non-clinical studies.
URI: http://hdl.handle.net/10314/4178
ISSN: 1759-9679
Aparece nas Colecções:Artigos em Revista Internacional (ESS)

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